Bilayer Lipid Membrane Formation by Electrowetting-Actuated Encapsulated Droplets

Abstract

We demonstrate the formation of a bilayer lipid membrane (BLM) by contacting two electrowetting-actuated encapsulated droplets across an aperture. The encapsulated droplets contained KCl cores surrounded by lipid/decane shells. A suspended BLM was formed at the contacting interface across an aperture between two encapsulated droplets. The BLM formation was reproducible, while the BLM remained over hours under optical and electrical inspections. An ion channel peptide, α-hemolysin, was incorporated into the BLM, showing the capability of electrophysiological studies of membrane proteins.

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